Diagnosis of Freemartinism Syndrome by Polymerase Chain Reaction (PCR) in Cattle

Polymerase Chain Reaction (PCR)

The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...

diagnosis of ornithobacterium rhinotracheale using polymerase chain reaction (pcr)

abstract: ornithobacterium rhinotracheale (ort) has been identified as one of the emerging respiratory bacterial pathogens in turkey and chicken flocks. diagnosis of ort infection has been faced some difficulties in isolation and identification of the bacterium based on the biochemical properties. a reliable identification method that can be used in routine laboratory investigations is of impor...

Polymerase Chain Reaction for the Diagnosis of Tuberculosis

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

sensitive detection of giardia cysts by polymerase chain reaction (pcr)

giardia is one of the most common human parasites and causes a lengthly course of nonbacterial diarrhea. disease outbreaks due to giardia infection are often attributed to contaminated water supplies. a major problem associated with detection for this organism is the lack of sensitive and reliable methods. pcr has the potential to address many of the limitations.we have performed a pcr-based me...